Dados do Trabalho

Título

DISCOVERY OF ANTIOXIDANT ACTIVITY IN PROTEIN HYDROLYSATE FROM AMAZONIAN BIOMASS FROM TUCUMÃ (Astrocayum vulgare)

Introdução

Amazon biome comprises feedstock as oilseeds, which are used commercially for oil produces and generates a biomass (underutilized by industry), that can be used to produce high-value products such as vegetable proteins, which have been correlated to their functionality due to their composition of bioactive peptides (with diverse biological functions). The aim of this study was the reuse of tucumã biomass (Astrocayum vulgare), extracting its protein to obtain peptide-rich hydrolysates and evaluate its antioxidant capacity (AC).

Material e Métodos

Protein of tucumã biomass was extracted in a mass/volume ratio of 1:10 for 60 minutes with pH adjusted to 10.0 followed by isoelectric precipitation at pH 4.5. To obtain the protein hydrolysate 2 methods were used: 1- alcalase enzyme, from Bacillus licheniformis (≥ 0.75 Anson units/mL activity) in PBS buffer with pH adjusted in 8.5, temperature of the 50°C, and enzyme/substrate (E/S) ratio 1:50, 1:100, and 1:200 and hydrolysis time of 3 hours and, 2- commercial trypsin enzyme in water with pH adjusted in 7.5, 37°C for 24 h on same conditions of E/S ratio for alcalase. The resulting hydrolysate was heated in a water bath at 100°C for 20 min followed to ice bath to inactivate the enzyme and then centrifuged at 10,000×g for 20 min. Resulting supernatant was collected, freeze dried, and stored at -18°C. The hydrolysate protein content was determined by the Lowry method, high performance liquid chromatography was used to identify the peptide peaks of the protein hydrolysates and, AC measured by the DPPH and ABTS methods.

Resultados e Discussão

The best hydrolysis conditions were using the E/S ratio of 1:200 for alcalase and trypsin enzymes, resulting in a hydrolysate with ~25 and 57% protein, respectively. The highest AC was obtained for the hydrolysate with alcalase, presenting ~1,685 DPPH uM TE and, ~6,838 ABTS uM TE for g/protein, while the AC for the tryptic hydrolysate was ~584 DPPH uM TE and, ~2,685 ABTS uM TE g/protein.

Conclusão

This is the first report in the literature of obtaining tucumã protein hydrolysates with antioxidant activity. This result gives the processing chain of this oilseed the option of exploring it as an alternative bioinput with functional potential.