Dados do Trabalho

Título

Effect of vitamin D3 encapsulated in brewer’s spend yeast on the composition of the gut microbiota

Introdução

Nutrients have been studied for decades, demonstrating their potential and importance for human health. Vitamin D3 (cholecalciferol) is important for bone health and the regulation of the immune system. Most vitamin D3 is synthesized in the human body through exposure to sunlight. However, considering the growing concern about diseases such as skin cancer, exposure to sunlight has become increasingly cautious, making vitamin D3 supplementation more common and necessary. This study investigated the modulation of the intestinal microbiota using vitamin D3-encapsulated yeast (Saccharomyces pastorianus) in a batch culture system, focusing on changes in the levels of ammonium ion and short-chain fatty acid (SCFAs) and the genetic sequence of the microbiota

Material e Métodos

Samples were divided into four groups: control, plasmolyzed yeast, free and encapsulated vitamin D3. In addition to genetic sequencing, the concentrations of acetic acid, propionic acid, butyric acid and ammonium ions were measured to analyze changes in the microbiota composition.

Resultados e Discussão

In the control group, the average levels of acetic acid were 6.55 mmol/L, while in the vitamin D3 encapsulated were 14.23 mmol/L. The level of ammonium ions decreased from 193 ppm in the control group to 93.2 ppm in the plasmolyzed yeast. The results of the genetic sequencing showed significant changes in the phyla and genera of the microbiota. Among the genera analyzed, an increase in Bacteroides was observed following the ingestion of encapsulated vitamin D3. Bacteroides are essential for the intestinal microbiota, where their increase helps to modulate the immune system and promote a balance that contributes to a healthy microbiota.

Conclusão

These data suggest that supplementation with vitamin D3-encapsulated yeasts not only increases the production of SCFAS, especially acetic acid, and reduces ammonium ion levels, but also modulates the composition of the intestinal microbiota. The encapsulation of vitamin D3 in yeasts could be an effective strategy to improve SCFAS production, reduce toxic compounds such as ammonia, and promote beneficial changes in the intestinal microbiota.