Dados do Trabalho

Título

BIOACTIVE EXTRACTS OF NATIVE AMAZONIAN BEE CERUMEN (Scaptotrigona postica): DETERMINATION OF TOTAL PHENOLIC COMPOUNDS, FLAVONOIDS AND ANTIOXIDANT ACTIVITY

Introdução

Cerumen is the material resulting from the mixture of plant resins, wax and bee secretions, and is produced by most stingless bee species. It has structural functions within the nests such as building brood combs, honey pots, pollen, as well as being used to mummify intruders and preserve the hive's internal environment. Although the chemical and bioactive properties of the components produced by Apis bees have been extensively studied, there are still few reports on this material produced by stingless bees, especially species from the Amazon.

Material e Métodos

In this context, the present work aimed to prepare two different hydroalcoholic extracts with Scaptotrigona postica cerumen, collected in the municipality of Tomé-Açú (PA). The first extract was prepared using ethanol:water (70:30, v:v), in an ultrasound bath, for 30 minutes at room temperature (±25°C); and the second extract was prepared using methanol:water (60:40, v:v in a thermostated bath for 60 minutes at a temperature of 50°C. After the extraction processes, both extracts were centrifuged at 10,000 rpm for 20 min. The extracts were evaluated by analyzing total phenolic compounds (TPC), total flavonoids (TF) and antioxidant activity (AA) using the ABTS•+ radical method.

Resultados e Discussão

The TPC content was 3.79 ± 0.09 mgGAE.g-1 for the methanolic extract and 4.33 ± 0.05 mgGAE.g-1 for the ethanolic extract. For TF, the methanolic extract presented a result of 0.34 ± 0.01 mgQE.g-1 and 1.35 ± 0.08 mgQE.g-1 for the ethanolic extract. And finally, AA presented results of 49.01 ± 2.18 µMTE.g-1 and 65.99 ± 7.83 µMTE.g-1, for the methanolic and ethanolic extracts, respectively.

Conclusão

The TPC content was 3.79 ± 0.09 mgGAE.g-1 for the methanolic extract and 4.33 ± 0.05 mgGAE.g-1 for the ethanolic extract. For TF, the methanolic extract presented a result of 0.34 ± 0.01 mgQE.g-1 and 1.35 ± 0.08 mgQE.g-1 for the ethanolic extract. And finally, AA presented results of 49.01 ± 2.18 µMTE.g-1 and 65.99 ± 7.83 µMTE.g-1, for the methanolic and ethanolic extracts, respectively.