Dados do Trabalho

Título

EXPLORING THE POTENTIAL OF EXTRACTS PRODUCED FROM AMAZONIAN GRAPES

Introdução

The Amazon's natural resources are an immeasurable source of biocompounds. The fruit popularly known as Mapati, which comes from the Pourouma cecropiifolia tree found in northern Brazil (Amazonia and Acre), has a composition that has been little explored for technological and medicinal purposes. The present study was developed to investigate the
potential of extracts of the peel and seed of the mapati fruit by analyzing their bioactive compounds and verifying their biological properties in vitro models.

Material e Métodos

The total phenolic content was determined using the Folin–Ciocalteu method. The in vitro cytotoxicity was evaluated in
HEPG2, , and A549 cell lines. The cells were plated into 96-well plates, after 24h, the cells were treated with 100 μL of the extracts (1-200μg/mL), and incubated for 48h, subsequently, 10μL of MTT solution (0.5 mg/mL) was added, incubated for another 4h, then the supernatant was replaced by 100 μL of dimethyl sulfoxide, and the absorbance was measured. The in vitro antiplasmodial effect of the mapati seed extracts was analyzed in W2 (chloroquine-resistant) and 3D7 chloroquine-sensitive) strains. Parasites were incubated in 96 well plates containing the extracts, and culture medium. All cultures were synchronized, and aliquots were taken at 12hr rings, 24hr trophozoites, and 36hr schizonts and treated with different concentrations (3,5– 320 μg/mL) of extracts with the parasite suspensions (1% parasitemia and 2% hematocrit). After 48h, the culture was added to 100μL lysis buffer solution and 0.1 μL/mL Gold nucleic acid gel stain, and the microplates were read

Resultados e Discussão

The Folin-Ciocalteu test identified the total phenolic content of the samples (172.13mgGAE/g and 3 mgGAE/g extract), the extracts also had cytotoxic action for the cell lines (HEPG2 IC50= 152,4μg/mL for peel and 35,4μg/mL for seed, A549 IC50=
114,9μgGAE/mL for peel and 50,6μg/mL for seed), as well as for the 3D7 and W2 strains(3D7 IC50= 28,14μgGAE/mL for peel and 13,57μg/mL for seed and W2 IC50= 24,96μgGAE/mL for peel and 15,39μgGAE/mL for seed). These results point out the use of the bioactive potential of the mapati fruit and its compounds as antimalarial effects

Conclusão

These results point out the use of the bioactive potential of the mapati fruit and its compounds as antimalarial effects.