Dados do Trabalho

Título

JABUTICABEIRA LEAF PHENOLIC EXTRACT AS A POTENTIAL INHIBITOR OF CELL VIABILITY AND ADHESION IN CANCEROUS CELLS

Introdução

Jabuticaba (Myrciaria cauliflora) is a Brazilian fruit of great interest for its nutritional and functional properties. However, there is still little information on the use of its leaves, which have a high content of phenolic compounds. This work aims to evaluate the cytotoxic and antimetastatic potential of the phenolic extract of jabuticaba leaves (EFFJ) in both, the ileocecal adenocarcinoma tumor cell line (HCT8) and the human umbilical endothelial cells (HUVEC).

Material e Métodos

Cells were seeded in 96-well plates for viability assay and incubated for 24h. Then, the treatment with EFFJ was diluted in the medium at 5 to 100μg GAE/mL concentrations for 48h under 5% CO2 tension. After the treatment period, 10µl of MTT (3-(4,5-imethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; 5 mg/mL) was applied to all wells, and the cells were incubated for another 4h at 37°C. The medium was replaced by 100µL of DMSO/well, its absorbance was blocked at 570 nm in a spectrophotometer and the IC50 parameter (50% inhibition of cell viability) was obtained. To perform the cell adhesion assay, cells were treated with concentrations of 2.5, 5, and 10μg GAE/mL of EFFJ for 1h, seeded in a 96-well plate pre-coated with 60 μL of matrigel and incubated for 12h, for membership. Cells were washed with PBS and adhered cells were stained with 0.5% crystal violet for 30 min, solubilized with 1% SDS for 30 min, and quantified at 570nm.

Resultados e Discussão

EFFJ showed cytotoxicity to HCT8 (IC50=49.54μg GAE/mL) and HUVEC (IC50>100μg GAE/mL); in addition, HCT8 presents a potential antimetastatic effect, preventing cell adhesion by 27.67% at a concentration of 2.5μg GAE/mL, 37.7% at a concentration of 5μg GAE/mL and 55% at a concentration of 10μg GAE/mL.

Conclusão

It is concluded that EFFJ is classified as potentially cytotoxic to HCT8, as it reduced cell viability by 50% at a concentration below 100μg GAE/mL, while HUVEC didn’t show a cytotoxic potential and the selectivity index was 2.02 (SI between 2 and 10), which shows that the extract had a better medium selectivity for cancer cells. The extract also potentially inhibited cell adhesion in possible cases of metastasis when tested on the cancer cell line (HCT8).