Dados do Trabalho

Título

ANALYSIS OF POTENTIAL ANTIMICROBIAL RESISTANCE IN BACTERIA ISOLATED FROM LETTUCE PURCHASED AT A STREET MARKET

Introdução

The street market is a vital location for selling a diverse range of food items. However, the majority of street markets do not possess adequate hygiene and sanitary standards for selling these items, which can result in the proliferation of
microorganisms. This study aimed to evaluate the resistance of bacteria present in lettuce sold at a street market in the city of Limoeiro do Norte, Ceará State.

Material e Métodos

The antibiogram was conducted using the disk diffusion technique on Mueller-Hinton agar with three bacterial
strains previously isolated from lettuce. Antimicrobial disks of imipenem, ciprofloxacin, azithromycin, and cefuroxime were added to each petri dish. After incubating for 24 h at 35 °C, antimicrobial sensitivity was determined by measuring the inhibition halos.

Resultados e Discussão

The results revealed that imipenem and azithromycin exhibited the highest antimicrobial potential, with 100% efficiency against the bacteria. In contrast, ciprofloxacin and cefuroxime showed 33.3% efficacy.

Conclusão

The development of high bacterial resistance to cefuroxime may be linked to the emergence of resistance to β-lactam antibiotics, the capacity to produce β-lactamase, and the acquisition of a new binding protein for penicillin, resulting in the loss of inhibitory capacity of the cell wall. Gram-negative bacteria naturally exhibit resistance owing to the reduced permeability of their cell walls and the expression of efflux pumps. Furthermore, mutations in bacterial topoisomerases
contribute to ciprofloxacin resistance. This study highlights bacterial resistance as a significant public health issue as well as an opportunity for the development of industrial products with antimicrobial potential.