Dados do Trabalho

Título

DEEP EUTETIC SOLVENT COMBINED WITH GAMMA RADIATION TECHNOLOGY: A PROMISING INTEGRATED EXTRACTION STRATEGY FOR ANTHOCYANINS FROM APPLE

Introdução

Apples are one of the most widely consumed fruits in the world, and play an important role in the human diet due to their health benefits, including the presence of anthocyanins. The use of Deep Eutectic Solvents (DES) and the application of Gamma Radiation represent innovative approaches to the extraction and stabilization of anthocyanins. DES are a sustainable and efficient alternative to conventional methods, and gamma radiation is a promising technique to preserve the properties of anthocyanins. In this work, DES were combined with gamma radiation to establish an efficient method for obtaining anthocyanins from apple epicarp.

Material e Métodos

The extraction of anthocyanins from the fresh epicarp of Fuji Suprema apples was performed using three solvents: 70% ethanol acidified with HCl (1%); DES 1: choline chloride (ChCl) and propylene glycol; DES 2: ChCl and tartaric acid. The extracts were exposed to gamma radiation at doses of 0.5 and 1.0 kGy, and the control was not applied. The stability of the extracts at temperature (60ºC, 70ºC, 80ºC and 90ºC) was analyzed in relation to the content of total monomeric anthocyanins (TMA) by the differential pH method and the results were expressed in mg/g cyanidin-3-glucoside (CG3) of dry mass.

Resultados e Discussão

The irradiated extracts had a higher TMA content, resulting in anthocyanin content of up to 14% compared to the non-irradiated extracts. The DES 1 extract irradiated at 0.5 kGy showed the highest concentration of TMA (4.69±0.45), being more stable to degradation at 60ºC. At the temperature of 70ºC, the DES 2 extract irradiated with 1kGy was more stable compared to the other extracts, presenting the highest concentration of TMA (1.71 ±0.15). At temperatures of 80ºC and 90ºC, anthocyanins were almost completely degraded in all extracts.

Conclusão

The extraction with DES proved to be an excellent alternative to conventional solvents, with biodegradable and ecologically correct characteristics, as well as the application of radiation, which did not present negative effects, evidencing its potential for use in the food industry.