Dados do Trabalho

Título

EVALUATION OF ULTRASOUND EXTRACTION COMBINED WITH DIFFERENT DEEP NATURAL EUTETIC SOLVENTS FOR RECOVERY OF PHENOLIC COMPOUNDS FROM ACEROLA RESIDUE

Introdução

In recent decades, economic and environmental problems have caused growing concerns with the large volume of residue generated in the food industries. The waste contains large amounts of bioactive compounds but is discarded into the environment. The industrial processing of acerola (Malpighia emarginata D.C.) produces enormous amounts of waste, which is generally discarded or undervalued. Green extraction is an alternative for recovering bioactive compounds from waste and is also a recent goal in the development of scientific and industrial research, and the development of environmentally friendly solvents for the extraction of active ingredients is urgently needed. Given the above, the present study aimed to evaluate the ultrasound extraction of phenolic compounds from acerola residue using 18 different combinations of natural deep eutectic solvents (NADES).

Material e Métodos

The acerola residue was obtained from a fruit pulp industry located in Recife and dried in an oven with air circulation at 60 ºC for 24 hours. Then the acerola residue flour was sieved and ultrasound extraction combined with NADES was carried out. To obtain the extracts, 1.0 g of acerola residue with 40 mL of solvent (NADES-water 50%/50%) was subjected to an ultrasonic probe, using a power of 600 Watts, frequency of 20 KHz, and time (15 min). Total phenolic content (TPC) was determined using the Folin-Ciocalteau reagent and the results were expressed in mg GAE/g.

Resultados e Discussão

The TPC of acerola residue flour ranged from 28.07 ± 0.36 to 50.38 ± 0.20 mg GAE/g. An extraction was carried out using only water as solvent (15.47 ± 0.28 mg GAE/g. It was observed that regardless of the combination of eutectic solvents used, the retention of phenolic compounds was superior to the extraction that used only water as a solvent. The formulation of eutectic solvents that showed the highest recovery of phenolic compounds was the one that used choline chloride (hydrogen bond acceptor) and oxalic acid (hydrogen bond donor), in a ratio of (1:1).

Conclusão

Ultrasound extraction combined with NADES presented a form of green extraction, which leads to the production of a potential ingredient for use in food, pharmaceutical and cosmetic products.