Dados do Trabalho

Título

EFFECT OF BLUEBERRY EXTRACTS ON BODY WEIGHT AND FOOD CONSUMPTION OF RATS SUBJECTED TO AN INTESTINAL MODEL OF DYSBIOSIS

Introdução

The extraction of phenolic compounds (PC) from blueberries (Vaccinium sp.) in an ecofriendly way using Natural Deep Eutectic Solvents (NADES), promotes increased bioavailability of PC. Considering the prebiotic effects of PC, we hypothesized that blueberry PC have beneficial effects in a model of intestinal dysbiosis and this effect may be potentiated by the use of NADES. This study aimed to assess the effects of blueberry crude extract obtained using NADES (CE-NADES) or organic solvents (CE-SORG) in body weight and water and food consumption in rat model of intestinal dysbiosis induced by antibiotics (AB) (CEUA: 3015110219-UFSM).

Material e Métodos

Adult Wistar rats were divided into control (n=32) and dysbiosis (n=32) groups that received water or AB by gavage (2 mL/kg b.w.) for 10 days. Both groups were further divided into four subgroups (control, NADES, CE-NADES, and CE-SORG, n=8/group) that received NADES or blueberry extracts (10 mg of PC/kg b.w.) for 11 days, concomitant with AB administration. On day 12, rats were euthanized.

Resultados e Discussão

Body weight was significantly increase on days 2, 6, 8, and 10, compared with day 0 (p<0.05). NADES treatment resulted in lower body weight (289.8 ± 3.7g) compared to control and EB-NADES treatments (305.1 ± 4.5g and 304.9 ± 4.0g, respectively) (p<0.05). Water consumption was affected by an interaction between dysbiosis and time (p<0.05), as animals in the dysbiosis group consumed more water than the control group. Regarding food consumption, there was an interaction between the dysbiosis and blueberry treatment (p<0.05), with a reduction in food consumption in the control group treated with CE-SORG (17.6 ± 0.7g) compared to control (24.0 ± 0.3g) (p< 0.05). The same was observed of CE-NADES (CE-NADES: 20.7 ± 0.5g vs NADES: 23.5 ± 0.4g) and CE-SORG (CE-SORG: 20.6 ± 0.5g vs Control: 22.4 ± 0.5g) in the dysbiosis group.

Conclusão

We concluded that intestinal dysbiosis affects water and food consumption and that treatments with CE-NADES and CE-SORG have been shown to negatively affect these parameters. Our research continues to explore other effects of blueberry extracts in the studied model of intestinal dysbiosis.