Dados do Trabalho

Título

EVALUATION OF THE CYTOTOXICITY OF UNAUÉ HIE OxG HYBRID CRUDE PALM OIL NANOPARTICLES WITH JACKFRUIT BY-PRODUCTS FLOUR AS ENCAPSULANTS

Introdução

Hybrid Crude Palm Oil (HCPO) is characterized by high productivity, low production costs and nutritional benefits, such as increased levels of carotenoids and unsaturated fatty acids and vitamin E. However, to ensure the stability of its different components and applications in food, nanoencapsulation can be promising alternative. Despite the widespread use of conventional crude palm oil in foods, there are concerns about its safety at the nanoscale. In addition, adverse effects such as nanoparticle cytotoxicity have been reported for various materials. Therefore, this work aimed to characterize and evaluate the cytotoxicity of HCPO nanoparticles with jackfruit seed flour (SF-NP) and jackfruit axis flour (AF-NP) as encapsulants.

Material e Métodos

The HCPO nanoparticles were prepared by the homogenization method. HeLa cells (human cervical cancer cells) were cultured in Dulbecco's Modified Eagle's Medium (DMEM), and the cytotoxicity of HCPO, SF-NP, and AF-NP was evaluated using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric assay at different concentrations (2.5, 50, 100, 150, 200, 250 μg/mL). ). The treatments that resulted in lower absorbance readings (p < 0.05) compared to the negative control were considered cytotoxic. Relative viability (%) was calculated as the ratio between the absorbance of the samples and that of the negative control.

Resultados e Discussão

The different concentrations of AF-NP, SF-NP, and HCPO had no cytotoxic effects (p > 0.05) on HeLa cells. Furthermore, there was an increase in cell viability at all concentrations of both nanoparticles, except at concentrations of 50 µg/mL for the AF-NP (98.11%) and 250 µg/mL for the SF-NP (99.05%).

Conclusão

The increase in cell viability at most concentrations can be attributed to cell stimulation and increased metabolic activity caused by the AF-NP and SF-NP. The nanoparticles and free HCPO showed no cytotoxicity to HeLa cells. Proliferative effects on the cells were also promoted, increasing relative viability at most of the concentrations used.