Dados do Trabalho

Título

IN VITRO EVIDENCE OF THE IMMUNOMODULATORY POTENTIAL OF BRACATINGA HONEYDEW HONEY IN LPS-STIMULATED J774 MACROPHAGES

Introdução

Bracatinga honeydew honey (Mimosa scabrella Bentham) (BHH), holds designation of origin from the southern Brazilian plateau and presents preliminary evidence of inhibition of pro-inflammatory cytokines in murine macrophage models. Macrophages are immune system cells involved in several roles such as phagocytosis, immunomodulatory action - due to cytokine release, and polarization of their M1 phenotype (generating pro-inflammatory responses) to M2 (generating anti-inflammatory responses). The cascade of reactions inherent to inflammation resolution is complex, and it is assumed that BHH exhibits effects on multiple events, such as the conversion of M1 macrophages to M2, thereby contributing to inflammation resolution.

Material e Métodos

The phenolic composition by LC-DAD, in vitro anti-inflammatory potential, and macrophage polarization capability of two BHH samples were investigated in J774 macrophages. After initial assessments of cell viability and nitric oxide release, BHH1 and BHH2 were tested regarding other cellular markers.

Resultados e Discussão

BHH1 amplified phagocytosis by 36.1 ± 1.8% (p < 0.001) and decreased cellular apoptosis by 58.9 ± 3.4% (p < 0.001). BHH1 also increased the expression of mannose-CD206, an M2 macrophage surface marker (anti-inflammatory), by 40.9 ± 0.8% (p < 0.05), while significantly reducing the surface marker TLR4-CD284 associated with M1 macrophages (pro-inflammatory) by 59.9 ± 4.1% (p < 0.001). The differentiation in effects may be associated with phenolic composition, as BHH1 had 6 distinct compounds identified (benzoic, salicylic, and protocatechuic acids, luteolin, naringenin, and rutin) totaling 96.6 μg/g, while BHH2 presented only syringic acid (19.95 μg/g).

Conclusão

This suggests the hypothesis that BHH1 plays an anti-inflammatory and immunomodulatory role, facilitating the transition of macrophages from the pro-inflammatory M1 state to the inflammation resolution M2 state. The resolution process interrupts inflammation, providing an additional opportunity for addressing diseases characterized by chronic inflammation, such as cardiovascular diseases, cancer, and diabetes. In this regard, these findings position BHH as a potential complementary strategy for treating inflammatory processes associated with these diseases.