Dados do Trabalho

Título

Can storage cause changes in the in vitro bioaccessibility of minerals in bracatinga honeydew honey (Mimosa scabrella Bentham)?

Introdução

Minerals act in various crucial biochemical pathways for human health. They are considered potentially bioavailable when released as ions after gastrointestinal digestion (bioaccessible). Research on bracatinga honeydew honey (BHH) – known for its higher mineral content compared to floral honeys, especially K – indicates that the minerals K+, Mg2+, and Ca2+ are potentially bioaccessible (> 90%). However, other scientific evidence has shown that storage time and temperature of BHH generate variations in the concentration of these constituents, which can influence the bioaccessibility. Therefore, the aim of this study was to evaluate the effect of storage on the in vitro bioaccessibility of K+, Ca2+, and Mg2+ in BHH.

Material e Métodos

For this, a sample produced in Lages (Santa Catarina, Brazil) was fractionated and the portions stored for 24 months at 23°C; 4 months at 40°C; and immediately frozen at -20°C (initial time). All treatments underwent a static in vitro gastrointestinal digestion model (gastric and duodenal stages). The cations K+, Ca2+, and Mg2+ were analyzed in duplicate using an Agilent Technologies capillary electrophoresis system equipped with a diode array detector. Analysis of variance (ANOVA) and Tukey's test (p>0.05) were the statistical evaluations applied.

Resultados e Discussão

At the initial time, the bioaccessible fractions for K+, Ca2+, and Mg2+ were 81.1%, 117.3%, and 106.4%, respectively, demonstrating the influence of in vitro digestion on the free mineral content. After honey storage for 24 months at 23°C and 4 months at 40°C, no significant changes were observed in the bioaccessible fraction of K+ (reduction of 1.5% and increase of 1.4%, respectively). However, these storage conditions caused reductions in the bioaccessibility of Mg2+ (4.5% and 8.0%, respectively) and increases for Ca2+ (27.0% and 35.4%, respectively).

Conclusão

Electronegativity (Mg > Ca > K), complexation forms, and changes in the studied matrix (after in vitro digestion and storage) are factors that can be associated with the distinct bioaccessible behaviors observed. Therefore, it is suggested that the storage of BHH impacts the in vitro bioaccessibility of its mineral constituents.